212 Manual of Veterinary 3Iicrohiology. 



Water 100 



Carbolic acid .... 4 



Hydrochloric acid ... 2 



Cultures — Cultures on artificial media succeed well 

 at temperatures varj'ing from 18° to 45°, 



Bouillon becomes turbid without showing any spe- 

 cial characters. 



Gelatin, inoculated in superficial lines, shows a 

 raised growth of white or bluish- white appearance, 

 with irregular, often lace-like, borders ; the medium 

 is not fluidified. Inoculated by puncture the culture 

 shows itself in the form of rounded colonies covered 

 with crystalline' projections. 



The culture upon potato is remarkable for its clear 

 brown color, which gradually becomes deeper with 

 age. 



Sowings ought to be taken from the parenchyma- 

 tous organs : liver, lungs, lymphatic glands, or from 

 the blood. 



Schweinitz has isolated from cultures a toxic 

 ptomaine (sucholo-toxin) and a special albumin (such- 

 olo-albumin). 



Hesearch and coloration. — Salmon's bacillus readily 

 takes up the difterent aniline colors; simple methods 

 of staining are alone applicable : hydro-alcoholic solu- 

 tions, Loifler's blue, etc. The methods of Gram and 

 Weigert, and Kiihne's violet completely fail. 



Experimental inoculations. — The disease is inoculable 

 to the mouse, rabbit and guinea pig. The pigeon 

 takes it only from large doses. 



In the mouse the microbe assumes larger proportions 

 than in the pig, and multiplies abundantly. 



The rabbit, inoculated under the skin, succumbs in 



