"228 Manual of Veterinary Microbiology. 



near the boiling point before the introduction of the 

 specimens to be stained. The necessary staining is 

 thus obtained in a few minutes. 



2. Lubimoff's method. 



The staining fluid is composed of-. 



Water .... 20 cub. cent. 



Boric acid . . . 0-5 grams. 



Absolute alcohol . . 15 cub. cent. 



Fuchsin . . . . 0-5 grams. 



This fluid keeps indefinitely. 



To stain the bacilli upon the cover glass a few drops 

 of the solution are deposited upon its surface and 

 heated for two minutes over a spirit lamp ; the 

 preparation is then rapidly decolorized in sulfuric 

 acid diluted to 1 to 5, then rinsed with alcohol and 

 immersed in a concentrated alcoholic solution of 

 methylene blue ; it is then washed with water, dried 

 and mounted in balsam. 



Sections of tubercular tissue are left during one or 

 two minutes in the staining fluid, previously heated 

 to near the boiling point, then passed for a few seconds 

 into alcohol and thence, for one to two minutes, into 

 a 1 to 5 dilution of sulfuric acid, again into alcohol 

 and, finally, for one minute, into a hydro-alcoholic so- 

 lution of methylene blue. Dehydration is accom- 

 plished by passing the sections through absolute alco- 

 hol and xylol and they are then mounted in balsam. 



3. Ziehl-Neelsen incthod. 



The staining fluid is composed by mixing : 

 Fuchsin . . • . . 1 gram. 



Absolute alcohol. .... 10 grams. 

 Five per cent aqueous solution of car- 

 bolic acid. .... 100 grams. 



