Microbic Diseases Individually Considered. 229 



The different steps of the operation are exactly the 

 same as in Lubimoff 's method. 



4. Herman's method. 



The staining fluid is made extemporaneously and 

 from two solutions : 



(1) A one-per-cent aqueous solution of carbonate 

 of ammonia ; 



(2) An alcoholic solution of methyl violet 6 B (1 of 

 the violet to 30 of 95 per cent alcohol). 



A few drops of the second solution are added to 

 several cubic centimeters of the first until the mixture 

 obtains a deep violet color and this is brought to a tem- 

 perature approaching ebullition. The cover glasses or 

 sections are left in the stain from one to two minutes, 

 then placed for from two to three seconds in nitric 

 acid diluted 1 to 4 for sections and 1 to 10 for cover 

 glasses ; after passing through the dehydrating fluids 

 they are ready for mounting. 



Double staining may be obtained by immersing the 

 preparations, after passing through the nitric acid and 

 the alcohol, in an aqueous or alcoholic solution of eosin. 



5. Kitfs inethod. 



This requires two fluids both of which preserve 

 themselves indefinitely : * 

 (1) Aniline water, .... 100 grama. 

 1 per cent solution of caustic soda, 1 " 



® [The aniline-containing solutions will generally be found unfit 

 for use after one or two weeks.— D.] 



