258 31anual of Veterinary Microbiology. 



of double staining are therefore inapplicable. Gen- 

 erally we have recourse to Loffler's blue, decolorizing 

 with carbolated water, at 1 to 300. 



Kiihne also recommends the following method of 

 staining: Sections, w^ell freed from alcohol, or cover 

 glasses, are placed in a solution composed of: water, 

 100; carbolic acid, 5; alcohol, 10; methylene blue, 

 1-5. They are decolorized by a rapid passage 

 through water acidulated with hydrochloric acid, and 

 washed in distilled water. The sections are dehy- 

 drated by a short immersion in alcohol, then placed 

 in aniline oil to which has been added a few drops of 

 oil of turpentine, then in pure turpentine, and, 

 finally, in xylol. 



The bacillus of glanders is difficult to demonstrate 

 in old lesions, where it appears to break up into 

 granules (perhaps into spores); it is always much 

 more abundant in acute lesions. 



Experimental inoculations. — Solijoeds, the sheep, goat, 

 pig, dog, cat, rabbit, guinea pig, field mouse and pig- 

 eon take the disease by inoculation. The ox, white 

 mouse, rat and chicken are refractory. The ass 

 always contracts acute glanders by inoculation. 

 When the virus is inserted by scarifications in the 

 forehead, for example, an extensive ulcer, with indu- 

 rated and inflamed borders, develops on this region. 

 Along with this the manifestations of the general 

 disease appear about the third day, and the subject 

 quickly dies. The autopsy reveals an eruption of 

 small reddish nodules on the respiratory mucous 

 membrane, pysemic infarcts in the lungs, liver, kid- 

 neys, spleen, marrow of bones, etc. 



In the dog the insertion of the virus in the skin or 



