2()4 Manual of Veterinary Microbiology. 



of giant cells; besides this marked tendency to sup- 

 puration, should be mentioned the early retrogressions 

 which lead to the ulcerations characteristic of the dis- 

 ease. 



Vaccinations. — Glanders leaves behind it no immu- 

 nity against another attack. Thus, auto-inoculations 

 and re-inoculations performed upon animals already 

 affected with the disease are followed by positive re- 

 sults. In the glanderous guinea pig these inoculations 

 determine local symptoms as intense and a generali- 

 zation as complete as at the time of a first insertion; 

 in the dog the second attack is also generalized and 

 often as severe as the first. According to Galtier the 

 dog can contract the disease as often as five times, 

 but the local symptoms become less and less marked 

 In the horse re-inoculations and auto-inoculations 

 may prove abortive or may produce a chancre with 

 or without lesions of the lymphatics, but fever and ag- 

 gravation of symptoms are never observed, the virus 

 seeming to limit its effects to the point of inocula- 

 tion. In the ass secondary inoculations are followed 

 by much more intense reactions; each insertion pro- 

 duces a marked tumefaction and a corresponding 

 ulcer, corded lymphatics, glandular engorgements — 

 in brief — the effects of a first inoculation. (Cadeac 

 and Malet.) 



The natural disease, therefore, does not confer im- 

 munity. Several attempts at artificial vaccination 

 have been made. Strans obtained immunity in a dog 

 by the intravenous injection of a small quantity of 

 virulent culture. A bei>ign disease is produced which 

 immunises the dog against intravenous inoculation of 

 large doses. 



