274 3Ianual of Veterinary Microbiology. 



tissues of the tumor may be stained red by picro- 

 carmine. 



The methyl violet, by this method, may act in two 

 degrees. When the preparation has been exposed to 

 the stainins: solution for a short time the mvcelium 

 of the actinomyces is alone colored violet, when it has 

 been longer exposed mycelium and conidia are stained 

 alike. 



Preparations of some value may also be obtained 

 by the employment of picro-carmine alone ; the fungi 

 are stained yellow, whilst the neoplasm is red, but 

 by this process we do not obtain a definition of the 

 structure of the parasite. 



MM. Cornil and Babes recommend double stainino- 

 by the method of Gram with eosin or safranin. 

 They thus obtain a violet staining of the central fila- 

 ments whilst the clubs or conidia are stainad red. 



Cultures. — Bostroem obtained cultures upon beef 

 blood-serum and upon agar, and has been able to 

 convince himself that the bright claviform enlarge- 

 ments of the periphery of the colonies are incapable 

 of multiplication, this property belonging to the cen- 

 tral filaments alone ; hence, he proposed classing the 

 actinomyces with the schizomycetes or bacteria. The 

 structure would be that of a cladothrix or branching 

 bacterium, and the swollen productions of the corti- 

 cal zone only incidental forms developing when the 

 parasite finds itself under special conditions of nutri- 

 tion. 



We have cultivated the actinomyces in alkaline 

 bouillons and our researches have led us to the same 

 conclusions. Inoculation of peptonized gl3'cerin- 

 bouillon with a particle of tumor produces a devel- 



