288 Manual of Veterinary Microbiology. 



only after three hours, 3 per cent carbolic solution 

 only after two hours. (Sanchez-Toledo.) 



In bouillon the development is very rapid ; the 

 liquid becomes turbid in one day and sets free small 

 bubbles of gas ; the growth abates and becomes de- 

 posited toward the fifteenth day. 



When a gelatin tube is inoculated by deep punc- 

 ture and kept at 18°, the growth forms, after four or 

 five days, small cloud-like points from which fine lines 

 radiate perpendicular to the puncture. The culture 

 has a floculent appearance, slowly fluidifies the gela- 

 tin, and bubbles of gas are disengaged; when the 

 gelatin is entirely fluidified the culture is deposited 

 in the form of white flakes. 



Cultures upon agar are less characteristic. Growth 

 also takes place upon serum and potato. Cultures 

 of tetanus emit a smell of burnt horn and produce 

 various gases, including carbonic acid and hydro- 

 carbons. 



Research and coloration. — The bacillus of tetanus is 

 easily stained with the aniline colors ; it takes the 

 Gram very well and appears as a slender rod, uniform 

 in size or swollen at one of its extremities. Before 

 the formation of the spore this enlargement stains 

 like the filament itself. The spores are not stained 

 by this process but may be colored by the method 

 usually employed for the staining of spores. 



Experimental inoculations. — The disease is inocula- 

 ble to the smaller animals and notably, with a sus- 

 ceptibility decreasing in the order in which they are 

 named, to the mouse, white rat, guinea pig, rabbit, dog, 

 pigeon and chicken. The mouse, white rat and guinea 

 pig are extremely susceptible, 0.002 cub, cent, being 



