Microbic Diseases Individually Considered. 289 



sufficient to produce in these animals the typical dis- 

 ease with fatal results iu 36 to 40 hours. The rabbit 

 requires 10 to 30 drops ; the symptoms appear from 

 the second to the third day and death occurs four to 

 ten days later. The dog, pigeon and chicken are 

 less susceptible and sometimes survive large doses. 



The inoculation succeeds well in the connective 

 tissue, in the peritoneum and in the arachnoid. In 

 the connective tissue it causes -an cedematous swell- 

 ing, the contractions appearing first of all in the ad- 

 jacent muscles. When inoculated in the peritoneum 

 or in the blood these appear similtaneously in all 

 parts of the body. The disease is not pro.duced either 

 by inhalation or ingestion of virulent products. 

 Ingestion of tetanic toxines is also without effect, 

 these toxines being destroyed by the digestive juices. 



According to the dose inoculated and the suscepti- 

 bility of the animals the experimental disease may be 

 acute or chronic, fatal or curable. 



In connection with experimental inoculations three 

 cases must be distinguished. The material to be in- 

 jected may consist of the entire culture, of the amor- 

 phous part alone freed from its microbes by filtration, 

 or, finally, of the microbes alone deprived of their 

 soluble products by filtration, lixiviation, or heat. 



In the first two cases — inoculation of the entire 

 culture or of its soluble products — the classic disease 

 is reproduced. In the last case, according to MM.Vail- 

 lard and Vincent, the inoculation remains without ef- 

 fect except when excessive doses are employed. The 

 tetanus bacillus, therefore, appears to be unable to 

 multiply in the organism in the absence of its toxines, 

 25 



