Microbic Diseases Individually Considered. 317 



be transferred to the culture medium, or the inocu- 

 lation made with the material obtained by scraping 

 the surface of the section. By sowing the latter 

 product on gelatin M. Arloing isolated four different 

 species, one being a bacillus {pneumo-bacillus lique- 

 faciens bovis) which rapidly fluidifies gelatin, and the 

 other three micrococci. Of the latter, one produces 

 white colonies resembling drops from a w^ax candle, 

 the second gives whitish colonies which become 

 wrinkled on aging, and the third, colonies which take 

 an orange yellow tint. M. Arloing attributes the 

 disease to the pneumo-baeillus liquefaciens bovis. The 

 four microbes inoculated separately under the skin 

 of a steer give an inflammatory tumefaction which 

 disappears in five or six days ; the largest tumefaction 

 is caused by the bacillus, and if several successive 

 generations are inoculated it ultimately happens that 

 the bacillus alone produces a local reaction. More- 

 over, the bacillus alone is constantly present in dis- 

 eased lungs. M. Arloing noticed that the isolated 

 effects of the microbes which he had cultivated re- 

 sembled only in a remote way those produced by 

 fresh serosity, but he observed, also, that this last 

 becomes more active in passing through the cellular 

 tissue of healthy cattle. By taking the microbes 

 from this reinforced serosity he obtained more viru- 

 lent cultures of the pneumo-baeillus ; 4 cc. of cul- 

 ture injected into the lung of one steer and 20 cc. 

 injected into the veins of another produced the speci- 

 fic lesions of pleuro-pneumonia. This author isolated 

 from cultures a soluble substances which possesses 

 remarkable phlogogenic properties, and which, of 

 itself alone, reproduces the characteristic inflammatory 



