Microbic Diseases Individually Considered. 321 



monary lobules have not the uuiform color seen in 

 the latter disease. We have observed that the pul- 

 monary lesions begin, as in pleuro-pneumouia, in the 

 interlobular connective tissue and, in the peripheral 

 lobules, progress toward the center. Peels has 

 noted, in calves affected with this disease, the fre- 

 quency of sero-fibrinous exudates and pleuritic ad- 

 hesions, lesions which have also been recorded by 

 M. Vanden Maeghdenbergh. He also mentions the 

 occasional occurrence of inflammations of the peri- 

 cardium, liver, kidneys, stomach and intestine. 



Microscopical examination shows, in the lung and 

 in the muco-pus of the bronchi, the presence of ovoid 

 microbes with rounded ends, measuring from Ip. to 

 1-5/i in length by 0-5/i in thickness, easily stained by 

 the aniline colors, but not stained by the Gram or 

 "Weigert methods. When stained with a very dilute 

 aqueous solution of gentian violet they fix the color 

 at their extremities, whilst the center remains clear. 

 They are motile, vegetate rapidly in bouillon and on 

 Bolid media, and are pathogenic for various species 

 of animals. 



The rabbit dies in twenty-four to forty-eight 

 hours after subcutaneous inoculation or ingestion of 

 cultures or virulent products. In the same animal 

 intra-pulmonary inoculation of a drop of culture pro- 

 duces a pneumonia. Death is a little later in the 

 guinea pig than in the rabbit. 



■^ Two calves, aged thirteen days and eight weeks, 

 and a one-year-old heifer, were inoculated by Poels, 

 the first in the right pleura, the second in the trachea, 

 and the third in the lung. They died after 20, 54, 

 and 66 hours, respectively, with lesions of septic 



