15 HISTOLOGY 



4. Tendon. Place on a slide a small piece of thin tendon from the end of one of the leg muscles of the 

 frog. Keep moist by normal salt solution. Hold one end in position with a needle and fray out the fibres 

 at the other end by passing a needle along them. Cover and examine fresh. Observe the white fibres in parallel 

 bundles with tendon cells jn rows between them. Irrigate with acetic acid (i %) and observe the nuclei of the 

 tendon cells. Notice that the fibres swell up into a gelatinous mass. 



5. Pigment cells. Take a piece of the web between the toes of a frog and spread it out on a slide. Observe 

 under the high power the pigment cells, some with numerous branched processes, others rounded, their processes 

 having been retracted. 



