16 HISTOLOGY 



6. Adipose Tissue. Take a piece of the mesentery of the rat and spread it out on a slide, mounting in 

 normal salt solution. Observe the spherical highly refractive fat-cells. Irrigate with a drop of osmic acid and 

 observe after half-an-hour that the fat has been stained black. 



7. Hyaline Cartilage. Strip off the free edges of any of the thin cartilages of the sternum of a frog. 

 Mount in normal salt solution and examine. Observe the cells lying in a matrix of collagen. Irrigate with 

 acetic acid (i %) and notice that the cells are more distinct and that their nuclei are now visible. 



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