17 HISTOLOGY 



8. Muscle Striated. Cut through the skin covering the thorax, and remove one of the strands of tissue 

 connecting the " sternum " with the skin. Lay it on a slide and tease out thoroughly. Crush some of the fibres 

 by pressing a bristle across them. Place on coverslip and let a drop of normal salt solution pass underneath. 

 Observe the muscle sheath of the fibres at the crushed spot and the alternate dim and light bands. Irrigate 

 with acetic acid (i %) and observe the scattered oval nuclei. 



* '. 



'v Hi" 



I/ 



9. Muscle Unstriated. Take a piece of the bladder of a frog and spread its outer surface on a slide 

 allowing its edges to dry and fix themselves to the glass. Rub off the internal epithelium with the finger. 

 Put on one drop of 90 % alcohol and then one drop of eosin and methylene blue. Examine under the high 

 power and observe the unstriated muscle fibres arranged in a plexus. Their nuclei are stained. Note also the 

 connective tissue cells with stained nuclei. 



