20 



AMCEBA 



Sketches of the animal to shew changes of shape : pseudopodia ; ectoplasm ; endoplasm ; contractile vacuole ; 

 food vacuoles; nucleus. 



Kill and stain with picrocarmine to bring out the nucleus. The introduction ot the staining fluid under the coverslip should 

 be effected by irrigation, i.e. by placing a drop of the fluid on the slide so that it just touches one side of the coverslip, and by 

 drawing out fluid from the opposite side by means of a piece of blotting paper. The staining fluid will then pass in to replace 

 the fluid taken up by the blotting paper. Care should be taken that there is always some fluid touching the coverslip, to replace 

 that taken out by the blotting paper. 



