370 Origin of Each Species Considered Separately. 



by enclosing them in bags. As a result of this, absolute 

 purity of the cultures was attained in 1894. A culture 

 of 440 which flowered during August and September 

 consisted entirely of dwarfs. 



I have not continued this experiment further because 

 it seemed to me more important to work with the mu- 

 tants themselves and to test the constancy of the first 

 generation. 



In 1895 I used for this purpose some nanellas which 

 had just arisen from the Lamarckiana-isimily, and from 

 a branch of it. I fertilized 12 of the former and 8 of 

 the latter with their own pollen excluding the visits of 

 insects from them. I collected the seeds of each plant 

 separately, sowed them in the following spring, and, 

 after a month, transplanted all the seedlings without ex- 

 ception, into wooden boxes, in manured soil, where they 

 would have plenty of room to develop into rosettes like 

 that shown in Fig. 79 A (p. 366). Some of them which 

 were too close together grew like the type shown in Fig. 

 79 B ; I removed the plants which surrounded these in 

 order that they might have room to expand their leaves 

 in. The recording, as a result of this, took place at dif- 

 ferent times but all during the month of June. 



The twenty seed parents of 1895 were raised from 

 the seeds of nine separate plants of Lamar ckiana of wdiich 

 five belonged to the third (p. 224) and four to the second 

 generation (p. 262). The twenty mutants themselves 

 therefore belonged to the fourth and third generations. 

 In the following tables I denote the grandparent by 

 Lam., the parent or mutant by Nan., and the seedlings 

 raised from the seeds of these latter by S. The letters 

 A-E refer to the five Lainarckiana-^A^ints of the third 

 generation, L-O to those of the second generation ; their 



