144 PATHOLOGY 



nodules and the glands of the thoracic cavity were swollen to an 

 enormous size. 



The third animal, which had received an injection into the trachea 

 with the tuberculous emulsion, died after 72 days. The post-mortem 

 examination revealed both thyroid glands hyperemic and swollen; 

 at the point of injection the trachea was the seat of a mass the size of 

 a pigeon's egg; the surface of this mass was covered with cothitless 

 miliary tubercles. The lungs contained similar miliary nodules, and 

 the right lung was even adherent to the pleura. The mesenteric 

 glands were normal. 



The remaining 12 calves were killed; three of them were more or 

 less tuberculous. The one which had inhaled 0.5 gm. pulverized 

 tubercle bacilli was killed after 259 days; the tuberculin reaction 

 before its death gave a doubtful result. The autopsy showed the 

 presence of a few very small nodules in the laryngeal mucous mem- 

 brane and of one nodule in the anterior wall of the left cardiac 

 chamber; this last one contained very many tubercle bacilli. 



The second animal had been injected with 1 cm. of the emulsion 

 from the tuberculous organs; it was killed after 256 days. The tuber- 

 culin reaction was positive before its death. The post-mortem exam- 

 ination showed the inguinal glands in the neighborhood of the point 

 of injection very much swollen; the liver contained a few nodules; 

 all the intraperitoneal glands were swollen, and some of them were 

 already the seat of cheesy degeneration. The lungs were normal. 



The third heifer had received an injection of 1 cm. of the emulsion 

 from a tuberculous lung into its abdominal cavity ; it was killed after 

 280 days. The tuberculin reaction before its death had also been 

 positive. The section revealed the peritoneum and liver to be the 

 seat of a small number of tubercles varying in size from a pea to 

 a small bean; some of them were cheesy. Both lungs were studded 

 with numerous grayish-white, hard miliary nodes. 



The other nine animals were found to be entirely free from tuber- 

 culosis. 



The five control animals were killed after from 217 to 364 days. 

 The autopsy showed four of them to be suffering from tuberculosis 

 and one to be free from it. 



If the above-mentioned results are considered collectively it will 

 be seen that from among 15 experimental animals six became tuber- 

 culous, while nine were demonstrated to be insusceptible. It is 

 further worthy of note that the changes in the infected organs were 

 relatively very slight. 



From a review of the entire experiment it can be seen that the 

 native Japanese bovines are to some extent susceptible to perlsucht 

 experimentally, but only if doses of tubercle bacilli are inoculated 

 so large as never to be received in the course of a natural infection. 



