JAPANESE CATTLE AND TUBERCULOSIS 145 



We can conclude from this that our native cattle show so little sus- 

 ceptibility to perlsucht that natural infection appears almost im- 

 possible. 



Experiment B. The same experiment was repeated on May 27 

 of this year; this time 33 native calves from 3 to 8 months old, and 

 weighing from 40 kilograms to 90 kilograms were employed. The 

 method of the experiment was exactly the same as in Experiment A. 

 To obviate too frequent repetitions these experiments will be 

 reported only briefly. 



Fifteen of the animals were infected intravenously; in 10 pure 

 cultures of perlsucht bacilli were employed, and in 5 the emulsion 

 from tuberculous organs; 8 were infected intraperitoneally (5 with 

 pure cultures and 3 with emulsion from organs); 3 were treated 

 with inhalations of pure cultures, while the last seven were infected 

 subcutaneously (5 with pure cultures, 2 with organ emulsions). 



Four mixed race animals were employed as control; in two of 

 them the injections were made intravenously (one with pure cultures 

 and one with organ emulsion); in the other two intraperitoneal 

 injections were given (one with pure culture and one with organ 

 emulsion). 



Before the experiment all of the animals were injected with tu- 

 berculin ; in none of them was a positive reaction obtained. 



Of the 33 animals, 7 perished in from five to 63 days after the 

 inoculation with the perlsucht bacilli, from a number of different 

 causes. Five of these animals showed some traces of the disease; the 

 other two were entirely free from it. 



The remainder of the 33 calves are still alive (August 10, 1904), 

 and apparently in the best of health. 



II. Experiments concerning the Susceptibility of Native Bovines and 

 of the Mixed Races to Human Tuberculosis 



The experiments were performed on 14 calves, of which 6 were 

 Japanese, and 8 belonged to the mixed types. Eight of them were 

 treated with pure cultures; 2 of them were given intravenous, 3 

 intraperitoneal, and 1 intratracheal injections; 2 were given in- 

 halations; the other 6 were treated with an emulsion made of the 

 organs of a man, whose death was due to miliary tuberculosis; the 

 organs contained numerous fresh tubercle bacilli; 3 were infected 

 intravenously, and 3 intraperitoneally. 



The tuberculin reaction before the experiment was negative in 

 all the instances. 



Two of the native animals, having had pure cultures injected 

 into the cervical vein, died after 30 days and 56 days. One of them 

 developed high fever eight days after the injection, this persisting 



