THE BROWN ROT OF SOLANACEAE I TECHNIC 



189 



and must continue to grow rapidly for at least several weeks. 

 Only disappointment will result from inoculations on old slow- 

 growing, woody plants, or with cultures which have been in the 

 laboratory for a considerable period. The best results may 



FIG. 131. Common sunflowers (Nos. 3 and 4) inoculated with Bacterium 

 solanacearum by needle pricks on July 12, 1918, at X (on the stem) and badly 

 dwarfed and wilting. The controls at either side were 33 inches high. Photo- 

 graphed July 30, 1918, about % natural size. Experiments of Smith and Godfrey. 



be expected from cultures isolated the same summer they are 

 used. 



Determine 



1. FOR THE ORGANISM. Morphology. Size in microns (stain 

 with methyl violet or Ziehl's carbol fuchsin), form, aggregation 



