348 BACTERIAL DISEASES OF PLANTS 



heat-sensitive, chloroform-tolerant in +15 peptone beef bouillon 

 (grows unrestrictedly), dry air-tolerant (30 to 50 days or more), 

 sodium chlorid-tolerant (to 6 or 7 per cent in +15 peptone 

 beef bouillon) non-coagulating (milk), casein translucing (milk 

 is cleared as by No. IV), rod-shaped, paired, clumped or 

 catenulate schizomycete, forming on the surface of +15 nutrient 

 agar-poured plates slow-growing, translucent, white, circular, 

 smooth, flat colonies, entire at first but becoming undulate- 

 margined after some days, and having for a time, like many other 

 colonies, a reticulate or striate internal structure. 



Growth on young agar-streaks moderate, white, odorless, 

 translucent, spreading, flat, dull to shining, smooth, becoming 

 finely granular; medium not stained. Contrast with No. IV. 



Colonies on +10 beef -bouillon peptone gelatin slow-grow- 

 ing, white, flat, circular to irregular with lobate erose margins 

 (compare with XIII). Growth in gelatin stabs filiform, best 

 at top; no stain, no liquefaction. 



Growth on Loffler's solidified blood serum, scanty to 

 moderate, yellowish white. No liquefaction not even after 

 many days. 



Growth on steamed potato moderate, spreading, flat, glisten- 

 ing, smooth, white to dirty-white (medium grayed, never 

 blackened), action on the starch slight. 



Growth in +15 peptonized beef broth always best at the 

 top where a pellicle develops which fragments easily on shaking 

 and sinks, forming a flocculent, turbid, odorless fluid (clear 

 after 3 months). 



Milk is not coagulated but becomes translucent (by some 

 change in the casein) ; it is then strongly alkaline and not 

 viscid. After 3 months and considerable evaporation the fluid 

 is gelatinous and somewhat brownish (near Saccardo's ochro- 

 leucous). Purple litmus milk becomes blue; there is never 

 any acid reaction (compare with No. IV). 



In Cohn's solution no growth or only a very slight growth 

 (contrast with No. XIII). 



In Uschinsky's solution a copious growth and a heavy 

 fragile pellicle, sinking readily. Fluid bluish-fluorescent (5th to 

 10th day), not viscid. Repeated in 1919 : uniformly well clouded 



