THE OLIVE TUBERCLE: CAUSE 401 



increase growth, and an alkaline reaction develops the same as 

 on plain litmus agar (even in 30 days there is no acid reaction) . 

 Experiment repeated in 1915 with the same result. Litmus- 

 mannit-agar first blues then becomes slowly purple, or red, 

 like the litmus-dextrose or litmus- galactose agar. Litmus- 

 glycerin agar remains neutral or nearly so for a week or more, 



I 



-r ,-,- 



w 



:. 



i'V: 

 t 



i 



V 



FIG. 309. Flagellate rods of Bacterium, savastanoi EFS, stained by van Ermen. 



gem's silver nitrate method. X 1000. 



and then becomes slowly purple, but never red: repeated 

 in 1915 with a good growth of the organism and the same result 

 (tubes under observation 67 days). 



Merck's peptone from flesh retards growth (Petri) ; prevents 

 all growth (Smith and Brown). Liebig's meat extract retards 

 growth (Petri). Beef bouillon is less favorable to growth than 

 Witte's peptone in water with saccharose (Petri). A little gas 

 is produced in the closed end of fermentation tubes in Uschin- 



26 



