MISCELLANEOUS: EXPERIMENTAL TERATOSIS 601 



There can be no reasonable doubt, therefore, that the tissues 

 which afterward proliferate not only lose water, but become more 

 acid. 



In a week or two all the root-injury has been repaired and 

 the plant takes on a new and vigorous growth and apparently 

 no harm has resulted. This new growth is, of course, from the 

 terminal buds which were shocked and the leaves and internodes 

 of which will exhibit copious proliferation when they develop. 

 As growth continues and the shoot elongates we soon come to 

 leaves and internodes formed after the date of the shock and 

 these will be free or nearly free from adventive shoots, like the 

 lower older tissues which were mature or semi-mature at the 

 time of the root-injury. Thus we may have on a large plant 



Acidity + 158 (Fuller's scale). The readings of the 3 titrations were 159, 157 

 and 158. 



II. 21 fresh terminal buds brought in, crushed, extracted, squeezed and filtered 

 as above: 



Acidity + 126 (three titra.tions, each of which gave the same reading). 



This experiment was repeated some days later (July 22-23) with the following 

 results : 



B. I. 9 tops (bearing 4 and 5 leaves each) cut away and left on a table for 24 

 hours: 



1. Fresh weight, 288 grams. 



2. Loss of weight in 24 hours, 44.5 grams. 



3. Juice of the 9 buds including uppermost small undeveloped red leaf extracted 

 from crushed tissues 10 minutes in hot water (90C., falling to 35C.), squeezed dry 

 in a hand press and filtered : 



Acidity + 180 (Fuller's scale). No reducing sugar present. It is a faintly 

 clouded pink liquid. 



II. Juice of 9 fresh buds treated in the same way: 



Acidity +168. No reducing sugar. The pink liquid is decidedly cloudy and 

 contains at least 10 times as much starch as I. 



C. I. In December, 1919, 24 rather slender shoots from side branches, each 

 bearing several leaves, were cut away, weighed and left in a heap in an open 

 wooden box on the hothouse bench f^r three days, the first two of which were 

 cloudy and wet: 



1. Fresh weight 242.25 grams. 



2. Loss of weight in 72 hours 59.75 grams. 



3. The 24 terminal buds were then removed, weighed, crushed (7 min.), ex- 

 tracted 8 minutes in 50 c.c distilled water at 90C. falling to SS'C., squeezed 

 dry in a hand press, filtered and titrated. The fluid was a cloudy pink which a 

 drop or two of the alkali changed to colorless: 



Acidity + 195 (Fuller's scale). 



II. Juice of 24 fresh buds treated in the same way: 



Acidity + 190. 



