COLORING AGENTS. 63 



ANILINE GREEN (Methyl Green) 



Has been recommended by Hanstein for staining 

 chlorophyll grains, to which it imparts a deep 

 green color, different from their natural tint. 



Treub J recommends it for staining the nucleus. 

 Nuclei which are not in process of division are 

 colored pale green. In those which are dividing 

 the so-called nuclear-plates assume an evident 

 green color. Strasburger 2 has used it for the 

 same purpose in combination with I per cent, 

 acetic acid. 



[For the use of iodine-green in double staining 

 cell-walls for convenience in class demonstrations, 

 see Ammonia Carmine.] 



MAGENTA. 



[Dr. Gibbs recommends magenta and chrysoidin 

 for staining consumptive sputum. Three fluids 

 are required: A Magenta crystals, 2 gm. ; pure 

 anilin, 3 gm.; alcohol (s.g. 830), 20 cc.; distilled 

 water, 20 cc. ; B A saturated solution of chrys- 

 oidin in distilled water, to which a crystal of 

 thymol has been added to prevent deterioration ; 

 C Dilute nitric acid (1:2). The sputum, after 

 being thoroughly dried on the cover-glass, is 

 floated on A for 15-20 minutes, then washed in 



1 Treub: Sur des cellules ve*getales & plusietirs noyaux. Archives 

 Neerlandaises, 1880, T. XV. ; pp. 7, 17 of the Separate. 



2 Strasburger : Zellbild. und Zdltheil., 3d ed., 1880. 



