142 MORPHOLOGY AND LIFE HISTORY 



in young specimens examined by you? What 

 do the annulus and marginal membrane repre- 

 sent? 



(d) Make drawings, not less than life size, of both a 

 young and a mature specimen, as seen from the 

 side, labeling all parts. 



(e) With a sharp scalpel or razor carefully divide 

 your specimen longitudinally through the 

 middle, and make a drawing illustrating all 

 features shown in longitudinal sectional view. 



.(/) Make a third drawing showing the structure 

 and outline of the stipe as seen in cross-section, 

 and a fourth drawing, showing the outline of the 

 gill as seen in cross-section. 

 D. Microscopic Characters: 



1. When suitable material is available, note and de- 

 scribe the mycelium, extending through the soil. 



2. The annulus and stipe. Mount (in clearing fluid 

 or water) thin longitudinal sections passing through 

 the stipe. Is the stipe composed of distinct tissues, 

 e.g., like the hypocotyl of Ricinus, or the thallus of 

 Fucus? If so, describe. Observe that the stipe is 

 composed of hyphae. Of what is the annulus 

 composed, and what is its relation to the tissue of 

 the stipe? Do the hyphae have cross-walls or 

 septa? If so, what angle do the septa make with 

 the walls of the hyphae? Do the hyphae branch? 



Do you find spaces between the hyphae? If 

 so, describe their size and distribution. Make a 

 drawing to show the features observed under 2. 



3. The pileus. Mount (in clearing fluid) a thin longi- 

 tudinal section passing through the stipe, pileus, and 

 a portion of a gill. Examine under low power. 

 Can you trace the hyphae of the stipe into the pileus? 



