CONTENTS. 



List of References vi 



List of Apparatus vii 



Laboratory Rules viii 



PART I. GENERAL BACTERIOLOGY. 

 CHAPTER I. MORPHOLOGY AND ELEMENTARY TECHNIQUE. 



EXERCISE. PAGE. 



I. Cleaning Glassware 2 



II. Plugging Flasks and Tube? 2 



III. Sterilization of Glassware 2 



IV. Preparation of Bouillon 4 



V. Filling Test-tubes and Flasks with 



Culture Media 6 



VI. Sterilization of Culture Media 8 



VII. Preparation of Gelatin 10 



VIII. Preparation of Agar 10 



IX Preparation of Potatoes 12 



X. Preparation of Water-blanks 12 



XI. Care of Culture Media 14 



XII. Platinum Needles 14 



XIII Test-tube Cultures 14 



XIV. Incubation of Cultures 16 



XV. Cleaning Slides and Cover-glasses. 18 



XVI. Preparation of Staining Solutions. 18 



XVII. Simple Cover-glass Preparation. .. 20 



XVIII. Use of Microscope 22 



XIX. Hanging-drop Preparation 24 



EXERCISE. PAGE. 



XX. Test-tube Cultures Illustrating 



Form Types 26 



XXI. Study of Test-tube Cultures 26 



XXII. Microscopical Study of Form 



Types 26 



XXIII. Drawing Bacteria 28 



XXIV Study of Cell Grouping 30 



XXV. Study of Involution Forms 30 



XXVI. Gelatin Plate Cultures 32 



XXVII. Agar Plate Cultures 34 



XXVIII. Roll Cultures 34 



XXIX. Study of Plate Cultures 36 



XXX. Use of Decolorizing Agents 36 



XXXI. Gram's Stain 36 



XXXII. Tubercle Stain (Gabbett) 38 



XXXIII. Staining Endospores 38 



XXXIV. Study of Endospores 40 



XXXV. Flagella Stain 40 



XXXVI. Capsule Stain 42 



CHAPTER II. PHYSIOLOGY OF BACTERIA. 



EXERCISE. PAGE. 



XXXVII Preparation of Special Media 44 



XXXVIII. Effect of Reaction of Media on 



Growth 44 



XXXIX. Effect of Concentration of Media 



on Growth 46 



XL. Effect of Temperature Variations 



on Rate of Growth 46 



XLI. Determination of Thermal Death 



Point 46 



XLII. Comparative Efficiency of Dry and 



Moist Heat 48 



XLIII. Effect of Desiccation 48 



XLIV. Effect of Chemicals on Bacteria . . 48 



XLV. Relation to Oxygen 50 



XLVI. Effect of Direct Sunlight 50 



EXERCISE. PAGE. 



XLVII. Detection of Gas 50 



XLVIII. Quantitative Analysis of Gas 50 



XLIX. Detection of Acids 52 



L. Quantitative Determination of Acids 52 



LI. Detection of Nitrites in Cultures. . 52 



LU. Detection of Ammonia 52 



LI 1 1. Detection of Sulphuretted Hy- 

 drogen 54 



LI V. Detection of Indol 54 



LV. Determination of Chemical En- 

 zymes in Cultures 54 



LVI. Variation in Enzyme Production. . 54 



LVII. Variation in Color Production .... 56 



CHAPTER III. TAXONOMY. 



PAGE. I 



Points to be observed in the study of Bacteria. . 57 I Classification of Bacteria (Migula). 



PAGE. 

 60 



CHAPTER IV. SYSTEMATIC STUDY OF REPRESENTATIVE NON-PATHOGENIC BACTERIA. 



EXERCISE. PAGE. 



LVIII. Preparation of Special Media 63 



LIX. Bacillus prodigiosus 64 



LX. Variety of Pigments 66 



LXI. Separation of Bacterial Coloring 



Matter 67 



EXERCISE. PAGE. 



LXII. Bacterium phosphorescens 68 



LXIII. Bacillus acidi lactici 70 



LXIV. Bacillus vulgaris 72 



(iv) 



