General Bacteriology. 59 



d. Character of whey: clear; turbid; flocculent. 



e. Reaction. 

 /. Gas. 



g. Odor. 

 8. BLOOD SERUM: (Same as streak cultures) . 



MORPHOLOGICAL CHARACTERS. 



a. Form. 



6. Cell grouping. 



c. Size. 



1. In terms of the inicromillimeter; breadth, average and extreme length- 



2. In terms of human blood cell. 



d. Stain. 



1. Aqueous solutions; stains easily or with difficulty; uniformly or irreg- 

 ularly. 



2. Special stain ; Gram; tubercle; etc. 



e. Motility. 



1- Brownian movement- 



2. Vital movement; sluggish or active; rotary or direct; most favorable 

 temperature; age; media; etc. 



3- Flagella; stained by Loeffler, Bunge or Van Ermengem's method; dis- 

 tribution, monotrichal, lophotrichal or peritrichal- 



/. Capsule; stained by Ziehl, Gram or Welch's method; most favorable con- 

 ditions; broad or narrow; present in serum, milk or on agar streaks. 



g. Spores; time required for formation; media; position in cell, center or end; 

 effect on shape of cell, clostridium, or drumstick; germination, time, temperature; stain, 

 Hauser or Moeller's method; temperature limits- 

 h. Vacuoles (plasmoloysis). 

 i. Crystals, 

 j- Involution forms- 

 k. Pleomorphism- 



1. Effect of various media. 



2. Effect of reaction of media- 



PHYSIOLOGICAL CHARACTERS- 



a. Effect of desiccation. 



b. Relation to temperature; minimum; optimum; maximum; thermal death 

 point. 



c. Relation to oxygen; under mica plate; in hydrogen. 



d. Relation to light; (Buchner's Experiment XL VI.). 



e. Relation to antiseptics and disinfectants 



/- Pigment production; relation of development to oxygen; relation of de- 

 velopment to character of medium; changes produced by alkali and acid; solubility; spec- 

 trum analysis. 



g- Gas production; rate, quantity and formula produced on glucose, lactose, 

 and saccharose media. 



