EXERCISE LXII. BACTERlUn PHOSPHORESCENS Fischer. 



GENERAL CONSIDERATIONS. Described by Fischer in 1887 (Zeitschrift fttr Hygiene, Baud II, p. 92). Found in Kiel harbor, dead 

 sea fish, oysters and occasionally on meat in shops. The production of light is shown in the dark, especially when the organism is 

 grown on a medium made by boiling two salt herrings in a liter of water, adding 100 gins, of gelatin to the filtrate without neutraliza- 

 tion, tubing and then sterilizing (Lehmann). Phosphorescence can even be restored to attenuated cultures by growth on this medium. 

 Inasmuch as oxygen is necessary to light production surface growths are best. 



REFERENCES. Lafar 160-164. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature : 



2. Relation to free oxygen: 



5. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.: . 

 4. Pigment production: 



5. Gas production in glucose media: 



a. Shake culture 



A. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours per cent., 48 hours . 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours 48 hours 



fecal odor; 24 hours 48 hours 



9. Enzyme production : proteoly tic 



. . . . (2) closed arm : 



per cent., 72 hours per cent., 



-.(5) gas formula, H : CO2 : : 



hours per cent. 



. to ammonia. 



. days, 

 days. 



. diastatic 



10. Characteristic odor. 



11. Pathogenesis 



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