EXERCISE I. Mil. BACILLUS ACIDI LACTIC! Hueppe. 



GENERAL CONSIDERATIONS. First described in 1884 by Hueppe in Mitteil. aus dem Kaiserl. Gesundlieitsamte, Bd. II, p. 1837. This 

 organism may be taken as a type of the bacteria causing sour milk. 

 REFERENCES. Lafar, 223-244. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature : 



2. Relation to free oxygen: '. 



3. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.: 



4. Pigment production: 



5. Gas production in glucose media: 



a. Shake culture 



4. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours percent., 48 hours. 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours 48 hours 



fecal odor; 24 hours 48 hours 



9. Enzyme production : proteolytic 



...(2) closed arm: 



. per cent., 72 hours per cent., 



...(5) gasformula, H : CO, : : 



hours.. 



percent. 



to ammonia.. 



days, 

 days. 



.diastatic. 



10. Characteristic odor., 

 it. Pathogenesis 



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