Name of organism . . 

 Source, habitat, etc. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature: 



2. Relation to free oxygen: 



3. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.: 



4. Pigment production: , 



5. Gas production in glucose media: 



a. Shake culture 



b. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours per cent., 48 hours. 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours 48 hours 



fecal odor; 24 hours 48 hours 



9. Enzyme production : proteolytic 



...(2) closed arm: 



.per cent., 72 hours per cent., 



(5) gas formula, H : COs : : 



hours., 



percent. 



to ammonia., 



days, 

 days. 



.diastatic. 



10. Characteristic odor. 

 u. Pathogenesis 



[78] 



