EXERCISE LXXVII. MICROCOCCUS HELTINESIS Bruce. 



This organism is the cause of Malta (ever and is found especially in the spleen of the diseased. 



REFERENCES. Bruce: Ann. de 1' Inst. Pasteur, 1899, 8; 239. Durham: Jour. Path, and Bact., 1898, 5; 377. H. 301 ; M. & E. 449. 



PHYSIOLOGICAL CHARACTERS. 



r . Relation to temperature :. . 



2. Relation to free oxygen: , 



3. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.: 



4. Pigment production: 



5. Gas production in glucose media: 



a. Shake culture 



b. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours per cent., 48 hours. 



(4) reaction in open arm; 



6. Acid or alkali production, litmus milk 



y. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours 48 hours 



fecal odor; 24 hours 48 hours , 



9. Enzyme production : proteolytic 



...(2) closed arm: 



.per cent., 72 hours per cent., 



...(5) gasformula, H : CO* : : 



hours., 



per cent. 



to ammonia.. 



days 



days 



.diastatic. 



10. Characteristic odor.. 



11. Pathogenesis 



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