Medical Bacteriology. 99 



The Micrococcus gonorrhoeae does not grow on the ordinary artificial media but may 

 be cultivated on the following: 



a. Blood agar. Blood drawn from the finger, under aseptic precautions, in a cap- 

 illary pipette is placed on the surface of agar either in tube or Petri dish. This blood 

 is then inoculated with the material containing the organism (pus or pure culture) and 

 smeared over the surface of the agar either with the loop or better by means of a sterile 

 camel's hair brush. 



6. Wertheim's method. Human blood-serum (from placenta or pleuritic or other 

 effusion may be used) in a fluid and sterile condition is placed in two or three test-tubes. 

 These are heated to 40 C. and inoculated with the material containing the organism, 

 making dilutions from one to another if necessary. To each tube is then added an 

 equal quantity of nutrient (ordinary or 2%) agar thoroughly liquefied and cooled to 

 40 C. The two are then thoroughly mixed and quickly poured into Petri dishes and 

 placed in the incubator at 38 C. Colonies appear in 24 hours. 



c. Rabbit blood-serum may be used either in a fluid or solid condition. 



