EXERCISE LXXXIX. BACTERIUM DIPHTHERIAS (Loeffler) Mig. 



BACILLUS OF DIPHTHERIA.; KLEBS-LOEFFLER BACILLUS. 



First described in 1883 by Klebs. First cultivated in 1884 by Loeffler. Found in the false membrane in cases of diphtheria and in 

 small numbers in spleen, liver, etc. ; occasionally in healthy throats. 



REFERENCES. Klebs: Verhandl. d. Kongressfuer innere Medizin, 1883, II. Loeffler: Mitth. aus dem Kais. Gesundheitsamte, 1884, 

 2; 421. A. 349; H. 162; L. & K. 207; M. & R. !i53; M. & W. 137; McF. 284; P. 329; S. 356. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature : 



2. Relation to free oxygen: 



3. Relation to other agents, such as: 



desiccation, light , disinfectants, etc.:. 



4. Pigment production: 



5. Gas production in glucose media: 



a. Shake culture 



b. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours percent., 48 hours. 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



y. Reduction of nitrates: to nitrites 



8. Indol production: 24 hours 48 hours 



fecal odor; 24 hours 48 hours 



9. Knzyme production : proteolytic 



...(2) closed arm: 



.percent., 72 hours percent hours. 



...(i) gas formula, H :CO, : : : 



per cent. 



to ammonia.. 



days. 



days. 



.diastatic. 



10. Characteristic odor. 



11. Pathogenesis 



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