EXERCISE XCVI. MICROSPIRA COMMA (Koch) Schroetei-. 



COMMA BACILLUS; CHOLERA VIBRIO. 



First described by Koch in 1884. Found in the intestinal contents of cholera patients and has also been isolated several times from 

 a water supply. 



REFERENCES. Koch: Berl. Klin. Wochenschr. 1884, no. 31 u. 32: A. 401; H. 244; L. & K. 181; M. & R 402; M. & W. 152; McF. 311; 

 P. 508; S 500. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature :. .. 



z. Relation to free oxygen: 



3. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.: . 



4. Figment production: 



5. Gas production in glucose media: 



a. Shake culture 



6. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours per cent., 48 hours 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours 48 hours 



fecal odor; 24 hours ,48 hours 



9. Enzyme production : proteoly tic 



....(2) closed arm: 



per cent., 72 hours percent., hours. 



..(5) gas formula, H : CO 2 : : : 



per cent. 



, to ammonia. 



. days. 

 days. 



. diastatic. 



10. Characteristic odor. 



11. Pathogenesis 



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