EXERCISE XCVII. MICROSPIRA flETSCHNlKOVI nig. 



VIBRIO METSCHNIKOVI. 



First described in 1888 by Gamaleia. Found in intestinal contents, blood and organs of chickens suffering from a disease resem- 

 bling chicken cholera. 



REFERENCES. Gamaleia: Ann. d 1' Inst, Past. 1888, 2; 482. A. 441 ; H. 256; M. & R. 436; McF. 332; P. 593; S. 511. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature : 



2. Relation to free oxygen: 



3. Relation to other agents, such as: 



desiccation, light , disinfectants, etc.: 



4. Pigment production: 



5. Gas production in glucose media: 



a. Shake culture 



b. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours per cent., 48 hours. 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates: to nitrites 



8. Indol production; 24 hours , 48 hours 



fecal odor; 24 hours 48 hours 



9. Enzyme production: protcolytic 



...(2) closed arm: , 



.percent., 72 hours percent., hours percent. 



...(5) gas formula, H : CO : : : 



to ammonia., 



days. 

 days. 



. diastatic. 



10. Characteristic odor., 



11. Pathogenesis 



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