Name of organism 

 Source, habitat, etc. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature: . 



2. Relation to free oxygen: 



3. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.:.. 



4. Pigment production: 



5. Gas production in glucose media: 



a. Shake culture 



b. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours per cent., 48 hours. 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours ,48 hours , 



fecal odor; 24 hours ,48 hours , 



g. Enzyme production: proteolytic 



(2) closed arm: 



.per cent., 72 hours per cent., 



, (5) gas formula, H : CO S : : 



. hou rs per cent . 



, to ammonia 



.days. 



.days. 



.diastatic . 



10. Characteristic odor 



11. Pathogenesis '. . 



[188] 



