Name of organism . . 

 Source, habitat, etc. 



References 



MORPHOLOGICAL CHARACTERS. 



SKETCHES. 



Form: 



a. Bouillon. 



*. Agar . . . . 

 c. Gelatin . . 



d. Other media 



2. Size 



3. Cell groupings 



and arrangements 



in growths 



4. Staining powers: 



a. Aqueous gentian-violet.. 

 *. Lot-filer's methylen-blue. 



c. Gram's stain 



d. Special stains 



5. Motility .' 



a. Character of movement . 



b. Flagella stain 



6. Spores 



7. Special characters, such as: 



deposits, vacuoles 



pleomorphic and involution forms, capsules, etc. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature :.. 



2. Relation to free oxygen: 



3. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.: 



4. Figment production: 



5. Gas production in glucose media: 



a. Shake culture 



b. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours percent., 48 hours 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours 48 hours 



fecal odor; 24 hours 48 hours 



9. Enzyme production : proteoly tic 



....(2) closed arm: 



per cent., 72 hours per cent 



..(5) gas formula, H : CO 2 : : 



, hours per cent. 



, to ammonia . 



. days, 

 days. 



. diastatic. 



10. Characteristic odor 



it. Pathogenesis '. ... 



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