EXERCISE Cl. BACILLUS OEDEMATIS Liborius. 



BACILLUS OF MALIGNANT OEDEMA. 



First described by Pasteur in 1877. Widely distributed in soil and putrefying material. Few cases on record of infection of man. 

 REFERENCES. Z. f. H., 1886; 1 : 158; A. 476; H. 302; L. & K. 305; M. & R. :!94; M. & W. 175; M. & W. 459; P. 543; S. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature: . 



2. Relation to free oxygen: 



3. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.: 

 4- Pigment production: 



i. Gas production in glucose media: 



a. Shake culture 



A. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours per cent., 48 hours. 



(4 ) reaction in open arm : 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours 48 hours 



fecal odor; 24 hours 48 hours 



9. Enzyme production: proteolytic 



(2) closed arm: 



.per cent., 72 hours per cent., 



(5) gas formula, H : CO S : : 



. . ..hours. 



per cent. 



. , to ammonia 



.days, 

 .days. 



.diastatic. 



10. Characteristic odor. 



11. Pathogenesis 



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