EXERCISE Cll. BACILLUS TETANI Nicolaier. 



Discovered by Nicolaier, 1884. First cultivated by Kitasato, 1889. Occurs in man and animals suffering from the disease and widely 

 distributed in nature, especially in soil. 



REFERENCES. Nicolaier : Deutszche Med. Wochenschrift, 1884; Kitasato: Deutsche Med. Wochenschrift, 1889; A. 469; H. 290; 

 L. & K. 230; M. & R. 376;M.&W. 171; McF. 274;P. 385; S. 482. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature :. 



2. Relation to tree oxygen: 



j. Relation to other agents, such as: 



desiccation, light, disinfectants, etc.: 

 4. Pigment production: 



5. Gas production in glucose media: 



a. Shake culture 



*. Fermentation tube, growth i:i: ui open .inn: 



(3) rate of development: 24 hours per cent., 48 luurs 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



j. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours , 48 hours 



fecal odor; 24 hours 48 hours 



g. Enzyme production : proteoiytic 



....(2) closed arm: 



. per cent., 72 hours per cetit., 



...(5) gas formula, H : CO^ : : 



.... hours. 



per cent. 



, to ammonia . 



. days. 

 . days. 



. diastatic . . . 



10. Characteristic odor. 



11. Pathogenesis 



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