Name of organism . . 

 Source, habitat, etc. 



PHYSIOLOGICAL CHARACTERS. 



i. Relation to temperature-.. 



2. Relation to free oxygen: 



3. Relation to other agents, such as: 



desiccation, light, disinfectants, etc:. 



4. Pigment production: 



5. Gas production in glucose media: 



a. Shake culture 



*. Fermentation tube, growth in: (i) open arm: 



(3) rate of development: 24 hours 



(4) reaction in open arm: 



6. Acid or alkali production, litmus milk 



7. Reduction of nitrates; to nitrites 



8. Indol production; 24 hours 



fecal odor; 24 hours 



9. Enzyme production : proteolytic 



per cent., 48 hours. 



(2) closed arm: 



per cent., 72 hours per cent hours. 



...(5) gas formula, H : CO 8 : : : 



per cent. 



, to ammonia. 



, 48 hours. 

 , 48 hours.. 



. days 

 . days . 



. diastatic . . . 



10. Characteristic odor 



11. Pathogenesis 



[160] 



