176 Medical Bacteriology. 



d- Wash thoroughly in water. 

 e. Dehydrate in absolute alcohol. 

 /. Clear in xylene and mount. 



Quick Method. 

 . Stain in carbol-methylen blue 10-30 seconds. 



b. Wash in distilled water. 



c. Tannic acid solution (10 %) ^1 minute. 



d. Counter-stain with & weak solution of eosin until sections are red. 



e. Wash in water until pink. 



/. Dehydrate in absolute alcohol. 



g. Clear in xylene and mount. 



Bacterium diphtheriae. Loeffler's or better Weigert's method. 



Bacillus typhosus. 



a. Loeffler's methylen blue or carbol-fuchsin 15 miu.-24 hrs. 



b. Wash slightly in distilled water. 



c. Place in 10% solution of tannic acid for 10-60 min. 



d. Dehydrate rapidly in alcohol. 



e. Clear in xylene. 

 /. Examine. 



g. Mount in balsam. 



Such sections examined under a low power will be found to contain heavily stained 

 masses, which under a high power prove to be clumps of bacilli. Not infrequently the 

 bacilli are difficult to detect in tissue from typhoid cadavers. 

 Bacillus suipestifer. Loeffler's method. 

 Bacterium Welchii. Weigert's and Loeffler's methods. 

 Bacillus chauvaei. Use Pfeiffler's stain: 

 a. Dilute carbol-fuchsin J4 hour. 



1). Absolute alcohol slightly acidutated with acetic acid until section is a reddish 

 violet tint. 



c. Xylene and examine. 



d. Mount in balsam. 

 Bacillus oedematis. Pfeiffer's stain. 

 Streptothrix actinomyces. 



a. Ziehl's carbol-fuchsin, 10 minutes. 



b. Wash in distilled water. 



c. Picri^ acid (cons. ale. solution). 



d. Wash in distilled water. 



e. Wash in alcohol (50%). 



/. Dehydrate in absolute alcohol. 

 g. Clear in xylene. 

 h. Balsam. 



Tissue stained yellow, rays red. 

 REFERENCES. M. & W. 239-286; N. 537. 



