198 Medical Bacteriology. 



B. OEDEMATIS. 



a. Make cover- glass preparations from fluid of affected parts- 



b. Also make anaerobic cultures. If material contains spores it should be heated 

 to 80 0. for 10 minutes before it is seeded- 



B. TETANI. 



a. Make cover-glass preparation from pus and search for drumstick bacillus. 



ft. Make cultures in glucose bouillon and agar-plates and develop in hydrogen. 



c. Inoculate animals with the discharge, and also with the bouillon culture, and 

 watch for characteristic symptoms. 



S. ACTINOMYCES. 



a. Place one of the minute sulphur yellow nodules in a drop of glycerine on a glass 

 slide and then apply gentle pressure. 



b. Even the low powers of a compound microscope will then show something of the 

 clustered arrangement which can be more carefully studied under a higher power. 



c. Intraperitoneal inoculation of guinea pig. One month later nodules on peritoneum. 

 AMOEBA COLI. CIX. 



REFERENCES, v. J. 405; Si. 514 and 518- See also texts under the various or- 

 ganisms. 



EXERCISE CXII. DIAGNOSIS OF RABIES. 



a. The medulla of the suspected animal is removed under aseptic precautions, as 

 soon as possible after death. In case the animal is some distance from the laboratory it 

 is best to cut off the head, pack it in ice and ship by express. 



ft. Place a piece of the medulla about the size of a pea, in 4 or 5 cc. of sterile bouillon 

 and thoroughly grind UD the same. 



c. Anaesthetize a rabbit with ether, clip the hair from between the eyes and ears 

 and disinfect with a carbolic acid solution. 



d. Make a longitudinal incision through the skin and subcutaneous tissue along 

 the median line, while a crucial incision is made through the periosteum on one side of 

 median line thus avoiding haemorrhage from the longitudinal sinus. The periosteum is 

 then pushed back and a disc of the skull (| inch in diameter) removed with a trephine 

 and the dura mater exposed. 



e. With a sterile hypodermic syringe introduce 2 or 3 drops of the suspension of 

 medulla beneath the dura mater, stitch the skin, disinfect, dry and seal the wound with 

 collodion. 



The rabbits apparently experience no inconvenience; the wound heals rapidly and 

 the rabid symptoms appear in from 15 to 30 days, although sometimes they may occur 

 earlier or much later. 



EXERCISE CXIII. EXAMINATION OF MATERIAL FROM HUriAN AUTOPSIES. 



At human autopsies smears from the organs should be made on cover-glasses and 

 afterwards stained and examined. Plate-cultures should also be made from the various 

 organs or instead parallel streaks over blood serum, agar-slopes or agar-plates. In all 

 eases the surface from which the material is to be obtained should first be burned to 

 avoid infection of cultures with extraneous germs. Portions of the various organs 

 should also be preserved and hardened in alcohol. 



