XII. MISCELLANEOUS. 843 



microscopical organisms at a constant degree of temperature 

 and moisture. (Exhibitor's construction.) 



Prof. Dr. F. Cohn, of the Institute of Vegetable Phy- 

 siology in the University of Breslau. 



This apparatus satisfactorily replaces the germinating apparatus of Nobbe. 

 The seeds are soaked for 24 hours in water, then 200 are placed in each 

 earthenware dish and covered over. The enclosed tin dishes are then filled 

 with water, which keeps in the porous clay dishes the moisture requisite for 

 the development of the seeds. The space between the double walls of the 

 chamber is now filled three-quarters full with water (the height of the water can 

 be seen by the glass gauge), and a small gas flame, governed by a Bunsen's 

 regulator and placed underneath the germinating chamber, keeps the tem- 

 perature very constant. This apparatus is used for the examination of the 

 germinating power of agricultural seeds, which takes place at the seed control 

 station (Samen-Controll Station) connected with the Institute of Vegetable 

 Physiology. Similar apparatus of different sizes are used in this station 

 continually for the culture of plants, especially microscopical growths, at a 

 constant temperature. They were employed especially by Prof. Cohn in his 

 researches on Bacteria. 



3935a. Cultivation Apparatus, with admission of air. 



The Imperial and Royal Pathological and Anatomical 

 Institute of the University of Prague (Director, Pro- 

 fessor Edwin Klebs). 



The circular vessel is filled to half of its height with a mixture of water, 

 glycerine, and sulphuric acid (or sulphate of copper), and the glass globe 

 dipped into the cohesive fluid. The little round vessel contains the substance 

 for cultivation, mostly isinglass gelatine. The open neck of the globe is 

 wrapped round with wadding, and on this is placed the small, broad-brimmed, 

 thick-shelled globe. The air in the globe, as well as the substrata of cultivation, 

 are purified by the infusion of a powerful jet of steam. The introduction of 

 germs, as well as the removal of samples, is effected under a " spray " of 

 permanganate of potash. 



Among 70 preparations by this method, only a very few showed a spon- 

 taneous development of fungi, whereas " Hyphomycetes " and " Schisto- 

 mycetes " thrive excellently in them. 



3935b. Apparatus for the cultivation of fungi. 



The Imperial and Royal Pathological and Anatomical 

 Institute of the University of Prague (Director, Pro- 

 fessor Edwin Klebs}. 



Two glass chambers for microscopical observation : 



a. Filled pearly full with isinglass gelatine. Implantation of " Microsporon 



septicum " of the year 1872. Ring-shaped progressive development of 

 the " Schistomycetes " round a capillary tube, which contained the 

 vaccine matter (or infection) until the year 1874. 



b. Filled half to its height with isinglass gelatine. The vegetation of the 



" Microsporon sept." ceased early, and the gelatine has to some extent 



become liquid. 



" Hyphomycetes " did not make any appearance, although they thrive in 

 such chambers. Without importation of germs, these chambers, with their 

 contents, will remain free from any formation of fungi. 



