Ill 



with the methods of working and the problems of ex- 

 perimental pathology. 



As appears from the following, one of us collected 

 during a space of two years a great amount of infor- 

 mation concerning the neutralisation of tetanolysin by 

 antitoxin, whereby a conception of this phenomenon 

 was obtained which was different from the previous 

 one. At the same time a great many investigations were 

 made into the ruemolytic action of bases, previously 

 used by Danysx as a help to investigation of haemolytic 

 toxins 1 ), and the curious action was discovered, which 

 salts of ammonia have upon ammonia in that respect. 

 These two phenomena seemed to be very like two 

 corresponding phenomena in physical chemistry - - the 

 phenomenon of neutralisation and the action of ammo- 

 nia salts upon the electrolytical dissociation of ammonia. 



Since we had obtained this result it seemed possible 

 for us to continue our work in this direction with the 

 hope of making still further progress. So last summer 

 we made a series of experiments together. The result of 

 these being not quite conclusive, we resumed them this 

 summer. Although we do not claim to have treated 

 exhaustively this exceedingly interesting subject, yet we 

 believe that we can offer a solution of some of the pro- 

 blems connected with it. In the present essay we shall 

 communicate the most important results of our investi- 

 gations and we hope that in the future, we shall be 

 able to continue our work and increase our experience 

 in this field. Some short papers on the results we have 

 obtained have already been read before the Royal Aca- 

 demy of Science in Stockholm (October 1901) and be- 

 fore the Congress of Naturalists at Helsingfors (July 1902). 



') Danysz: Contribution a I'etude de rimmunite. Proprk'tes des melanges des toxines 

 avcc Icurs antitoxines. Constitution des toxines. Annales de 1'Inst. Pasteur 1899. 



