Ill 



from horseblood immediately before the experiments 

 took place. The inorganic preparations such as bases, salts 

 and acids were of the best kind which could be pro- 

 cured, and they were tested by comparison with nor- 

 mal solutions. 



Experiments with varying quantities of active bodies. 



As an example of the above mentioned series of ex- 

 periments, the following may be cited, in which the 

 amount of blood was (nearly) constant and the amount 

 of toxin varying. The toxin used was partly a solution 

 of 1 part dried tetanolysin to 499 water, which we will 

 call simply 0,2% lysin - - and partly a 0,05 n solution 

 of ammonia. These solutions were added to 10 cc. 

 2,5 % blood. The result is shown in the following table, 

 where a signifies the amount of the solutions added, 

 expressed in cc., b the percentage of haemolysis; c and 

 (/ the calculated quantities, whose significance will be 

 indicated below. The lysin had been standing in solu- 

 tion for (5 days (in an ice safe), and had therefore 

 become about 3 times weaker than fresh lysin. 



The action of varying amounts of lysin upon 2,5 / blood for 1 

 hour at 37 C: 



a b c d 



1,0 45 0,91 7,4 



0,8 25 0,74 6,8 



0,6 14 0,57 6,6 



0,5 7 0,48 5,5 



0,45 6 0,43 5,7 



0,4 3,5 0,38 4,9 



0,35 4 0,34 5,9 



0,3 2,5 0,29 5,5 



0,25 2,5 0,24 6,6 



0,2 1,7 0,20 6,5 



0,17 1 0,17 5,9 



0,13 0,5 0,13 5,4 



n - 



