Ill 



It appears that the haemolysis increases rapidly with 

 increasing amounts of lysin. The irregular course of 

 the ciphers shows that the errors of experiments are 

 not insignificant. The haemolysis increases roughly 

 speaking, in proportion to the square of the concen- 

 tration c of the toxin; this is calculated after the for- 

 mula c -- = jjp^-, being directly proportional to the added 

 quantity a and inversely to the volume 10 -f a. If the 

 square root of b be divided by c, then the result is 

 nearly constant as indicated under d. Their mean would 

 be 6,1, and the deviations from this are so small that 

 they do not go beyond errors of experiment. Three 

 other series of experiments carried out with lysin about 

 the same time showed similar results. 



An experiment with 0.05 n ammonia gave the fol- 

 lowing results: 



The action of varying amounts of ammonia upon 2,5 % blood. 

 1 hour at 37 C: 



a b c d 



1,0 65 0,84 9,6 



0,8 55 0,67 11,1 



0,6 37 0,50 12,3 



0,5 27 0,40 12,8 



0,45 16 0,36 11,1 



0,4 12 0,31 11,1 



0,35 6 0,27 9,2 



0,3 5 0,22 10,2 



0,25 1,5 0,17 7,2 



0,2 0,7 0,12 6,8 



0,17 0,6 0,09 8,3 



0,13 0,4 0,05 11,6 



The result of these experiments is shown in fig. 1, in 

 which the dotted line represents the ammonia and the 

 straight one the tetanolysin. For ammonia c is calculated 

 from the formula c = - 5 '' b ecause lne ammonia 



- 12 - 



