Ill 



rences are quite within the limits of errors of experi- 

 ment. The calculation has been made, under the suppo- 

 sition that the fixed amount is in proportion to the 

 fixing amount of blood. Tha NaOH being 1/20 normal 

 and the NH 3 being 1/27 n, 0,6 cc. of the NaOH so- 

 lution is equivalent to 0,81 cc. NH 3 solution. It appears 

 from this that equivalent amount of NaOH and NH 3 

 will fix the same amount of blood. In the case of 2,5 % 

 of 10 cc. blood and 0,05 n alkali, the fixed quantity is con- 

 tained in 0,075 cc. of the alkaline solution. The experiment 

 seems to indicate that the combination of a base with the 

 blood corpuscles is of a rather solid character, the am- 

 monia combination not being apparently more hydro- 

 lysed than the sodium combination and the dilution ha- 

 ving no sensible influence upon the hydrolysis. Yet 

 several facts which will come to light later indicate that 

 such a hydrolysis exists, only not necessarily in such a 

 degree that it is of significance in this case. 



In the above calculation it was taken for granted 

 that the ammonia which was fixed to the blood corpus- 

 cles had no effect on their haemolysis but was, so lo 

 speak, removed from the reaction. The calculation shows 

 a fairly good agreement with the observed results, the 

 values under d being nearly constant. Little notice must 

 be taken of the weak concentrations, where the haemo- 

 lysis is below 2 %, for in these cases it cannot be de- 

 termined with exactitude. 



Also the tetanolysin is fixed to the blood corpuscles. 

 This combination seems to be of a less solid character 

 as we did not succeed in finding a sharp limit, where 

 the haemolysis ceased as in the case of NaOH and NH 3 . 

 The figures for tetanolysin have therefore not received 

 corresponding corrections. 



One would naturally think that a similar correction 



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