Ill 



The cases where tola! haemolysis occurs are signified 

 by an asterisk. To see what would happen with small 

 amounts of blood, when total haemolysis did not occur, 

 the following experiments were made, in which the 

 amount of alkali was varied together with the amount 

 of blood. In this case 0,01 n NaOH and 0,01 n NH 3 

 were used. 



The action of various amounts of blood upon various amounts 

 of NaOH and NH 3 . 



p 

 o 



p 



O 

 33 



3 



33 



From these data it appears that a greater (equivalent) 

 amount of ammonia than of sodium hydrate is re- 

 quired, to bring about complete haemolysis. It also ap- 

 pears that to bring about complete haemolysis of small 

 quantities of blood a relatively larger amount of sodium 

 is required than for larger amounts. 0,1 % blood dilu- 

 tion corresponds namely to 0,3 cc. of the NaOH solu- 



