Ill 



hydrate has. And the tetanolysin which combines with 

 the corpuscles at a still slower rate, has a straighter 

 curve than ammonia. With lysin there is therefore no 

 sharply defined point at which a strong hiemolytic ac- 

 tion suddenly takes place. 



As in the case of sodium hydrate, the amount of 

 lysin, required for complete hiemolysis, increased slower 

 than it would have done if the increase had been pro- 

 portionate to the increase of blood. While for complete 

 haemolysis of 0,5 % blood 0,4 cc. solution of lysin is 

 required, for 0.2% blood only 1 cc. (instead of 1,6 cc.) 

 was needed and for 2,5 % blood only 8 cc. (according 

 to another series of experiments). The quantity of toxin 

 required for complete htumolysis increases, roughly 

 speaking, in proportion to the square root of the amounts 

 of blood. 



The velocity of reaction of haemolysis. 



To penetrate a little further into the many curious 

 phenomena connected with the process of huemolysing, a 

 series of experiments was undertaken, dealing with the 

 velocity of reaction or, more correctly speaking, with 

 the time, in which Inemolysis proceeds to a certain de- 

 gree. For this object we used two different methods. 

 According to the first one, the lysin should during a 

 fixed time, and at a fixed temperature, act upon a blood 

 dilution 2,5%. Afterwards it should be cooled down, 

 centrifugalised and the strength of the htemolysis deter- 

 mined through the colour of the fluid. In this case much 

 stronger solutions of toxin can be applied than in the 

 usual case. We will explain the second method later on. 



In this case the surplus of toxin is so great, that it 

 may be considered as practically constant throughout 

 the whole process. As slated above, 10 cc. of 2,5% 



26 



