Ill 



tion of the limes can creep in, owing to the time taken 

 by the cooling process. 



In the case of sodium hydrate and x=6 the follo- 

 wing times 20,5 , 9,8 and 4,2 minutes are found for the 

 addition of 0,15 , 0,3 and 0.6 cc. of 0,5 n NaOH al 37 

 C. The calculated figures are (20,5), 10,2 and 5,3. The 

 concordance is satisfactory. 



What is said for ammonia will also apply to lysin. The 

 rate of reaction increases with the amount transformed. 



In the table below, the results of some experiments 

 at 20 C are quoted. The signs are the same as those 

 used above in the ammonia table. 



x = 7 13 20 30 



A 10 cc. blood -(- 0.3 cc. 0.2 "/ lysin 11 17 25 



B do. + 0.6 5(6.8) 7.5(9.0) 10.2(13.2) 15.5 



C do. +1.0 4(5.8) 5.9(8.5) 9.2(10.0) 



Supposing that no lysin is fixed by the corpuscles, 

 (a supposition which is certainly not true) the limes 

 ought to be in proportions of 1 : 0.53 : 0.442. As appears 

 from the values in parentheses (which are calculated 

 in this way) the reaction proceeds quicker at the higher 

 concenlralions lhan il would if regulated by proportio- 

 nality. The deviations are yet not so great, as not to allow 

 some doubt about the validity of the law of proportio- 

 nality in this case. We should be more correct in sup- 

 posing that a part of the lysin is fixed by the blood 

 corpuscles. Bui still also in the case of ammonia and 

 sodium, grealer amounts acl somewhat quicker than is 

 required by the law of proportionality. This deviation 

 probably depends on the method of experimentation. 



As well this circumstance as the chemical fixing are 

 effective in the case of lysin, therefore the deviation in 

 this case is greater than with the alkalies. 



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