Ill 



We can now get an idea of the causes of the above 

 mentioned rule, thai the amount of blood huemolysed 

 is proportionate to the square of the amount of toxin 

 (within certain limits). One might perhaps at first ex- 

 pect thas this would indicate that the velocity of reaction 

 should be proportionate to the second power oftheamount 

 of toxin, while we have just found that it was proportio- 

 nate to the first power. 



Let us take for example the series C in the case of 

 ammonia. From this it appears that a proportionality be- 

 tween the velocity of reaction K x and the square root 

 of the amount transformed, x, takes place. 



The following are the values: 



t --= 5 9 15 19 min. 



x = 8.5 20 43 5(5 /o 



K!= 0.0077 0.0107 0.01(53 0.0187 



10 a KI : Vx - 2.64 2.40 2.49 2.50 



Sodium hydrate (0,3 cc. of 0,05 n solution to 10 cc. 

 blood dilution) gives at 37 C the following values which, 

 if they do not follow the said rule as closely as those 

 in the table given above, still follow it, if we allow for 

 rather great errors of experiment. 



In the case of tetanolysin (0,6 cc. 0,2% lysin) at 20 

 C the following values are observed and calculated: 



10 3 K! 



31 



