Ill 



which examined during a certain time, (1 hour), seemed 

 lo he proportionate to the square of the active amount 

 of toxin, hut which is in reality proportionate to the 

 first power of it. We thought this case of sufficient in- 

 terest to justify the thorough analysis given above. 



The influence of temperature on the velocity of reaction 

 is an interesting question. In ordinary chemical proces- 

 ses, the velocity of reaction increases for each 10 C. with 

 values lying between the proportions 1.93 : 1 (in the case 

 of saponification of ethylacetate by strong bases) and 

 4.2 : 1 (in the case of inversion of cane sugar). 



In these experiments it was absolutely necessary to 

 see that the same sample of blood was used for the 

 higher and lower temperatures (2,5% blood was used). 

 The following data show the increase of the rate of 

 reaction. 



The figures show that the increase is the same for 

 ammonia and sodium hydrate, just as one would expect. 

 For tetanolysin it is a little greater. All three values 

 lie within the limits of the values for other chemical 

 reactions with which the haemolysis ist herefore related 

 also in this respect. 



Another matter of interest is the relative velocity of reac- 

 tion of sodium hydrate and ammonia. If it had been the hy- 

 droxylions which affected the blood corpuscles, one 

 would have expected that a certain amount of sodium 



